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 National Science
Foundation Award #0449183 |
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CAREER: A Framework for Directed Genome Evolution |
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| Investigator(s): |
Ryan Gill (PI)
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| Sponsor: |
University of Colorado at Boulder, CO 80309 3034926221
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| Start Date/Expiration Date |
2005-07-01 to 2006-06-30 (amended 2005-01-31) |
| Awarded Amount to Date: |
$78,431 |
| Abstract: 0449183
Gill
The proposed work entails optimizing protoplast fusion and genome-shuffling approaches in the bacterium, E. coli. Additionally, gene chips will be used to identify the genes responsible for antibiotic (aa-hydroxamate) tolerant phenotypes, and small network theory will be employed to understand how connectivity and clustering may affect antibiotic tolerance versus fitness. Improving the understanding of genome evolution and the tradeoffs between the yield from individual metabolic pathways and organism fitness is expected to make a positive impact on the metabolic engineering community. The impact follows from more insights being gleaned on how "global" versus "local" optimization is managed at the molecular level by cellular systems. Such insights, in turn, will allow for improved quantitative prediction of the outcome of different metabolic engineering strategies. Understanding better how genomic evolution can lead to antibiotic resistance and organism fitness should also positively impact work on contending with the medical problem of antibiotic resistant bacteria. The plan to integrate research into education and outreach has two objectives. First, outreach activities will be pursued via Women in Engineering, Multicultural Engineering, and high school honors programs. Secondly, undergraduates will be organized into teams that are charged with developing laboratory demonstration modules. Such teams will integrate prior coursework and learn collaboration skills while producing useful courseware. |
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| NSF Org: |
BES - Division of Bioengineering & Environmental Systems |
| Award Number: |
0449183 |
| Award Instrument: |
Continuing grant |
| Program Manager: |
Fred G. Heineken
BES Division of Bioengineering & Environmental Systems
ENG Directorate for Engineering
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| NSF Program(s): |
BIOTECHNOLOGY |
| Field Application(s): |
Industrial Technology |
| Program Reference Code(s): |
BIOPROCESSING/BIOMOLECULAR MATERIALS, 9181
FACULTY EARLY CAREER DEVELOPMENT PROGRAM, 1045
PECASE- eligible, 1187 |
| Program Element Code(s): |
1491 |
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