NIRT - Nanoscale Engineering of Bilaterally Accessible Biomembrane Mimics

Investigator(s):

Mathias Loesche (PI)

Sponsor:

Carnegie-Mellon University, PA 15213 4122688746

Start Date/Expiration Date

2005-10-01 to 2007-08-31 (amended 2006-01-10)

Awarded Amount to Date:

$736,426

Abstract: This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 02-148, category NIRT. The research focuses on the characterization and application of S-layer proteins, which are molecularly thin protein sheet crystals located on the outer cell envelope of a variety of micro-organisms. The crystal lattices incorporate nanoscopic pores located on repetitive lattice sites and can function as natural ultrafiltration devices. Reinforced S-layer lipid membrane hybrid architectures will be implemented on mesoporous, macroscopic substrates leading to functional, macroscopically-stabilized biomembrane mimics, accessible from both sides by small molecular species. Nanoporous gold will provide the interface to the macroscopic world. As a model of biomembrane functionalization, hemolysin will be assembled into the S-layer for use in measuring the passage of a single molecule. With these structures it may be possible to thread single-stranded DNA through the pores and to attach proteins to the ends of the DNA, thus engineering the capture and release of DNA from the pore. In terms of the broader impacts, these structures may find applications in a variety of devices including membranes and sensors. Collaborations with NIST and a transatlantic educational program are planned.

NSF Org:

CTS - Division of Chemical & Transport Systems

Award Number:

0555201

Award Instrument:

Standard Grant

Program Manager:

Eric S. Peterson
CTS Division of Chemical & Transport Systems
ENG Directorate for Engineering

NSF Program(s):

COLLABORATIVE RESEARCH, SEPAR & PURIFICATION PROCESSES

Field Application(s):

Program Reference Code(s):

CENTRAL & EASTERN EUROPE PROGR, 5979
EASTERN EUROPE, OTHER, 5915
NANO NON-SOLIC SCI & ENG AWD, 7237
NANOSCALE: INTRDISCPL RESRCH T, 1674
UNASSIGNED, 0000

Program Element Code(s):

7298
SEPAR & PURIFICATION PROCESSES, 1417