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 National Science
Foundation Award #9004561 |
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Crystal Structures of Limulus Hemocyanin
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| Investigator(s): |
Karen Magnus (PI)
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| Sponsor: |
Case Western Reserve University, OH 44106 2163684510
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| Start Date/Expiration Date |
1990-09-01 to 1993-08-31 (amended 1992-07-08) |
| Awarded Amount to Date: |
$272,000 |
| Abstract: The long term aim of this project is to determine at atomic resolution the three dimensional structures of various forms of hemocyanin, an oxygen transport protein found in invertebrates, with the intent of understanding the intricacies of oxygen binding by these copper-containing proteins. The specific objectives are: (1) the determination of the crystal structure of the oxygenated and deoxygenated forms of subunit II of Limulus polyphemus hemocyanin; (2) Comparison of the oxy and deoxy structures, based on data from a single crystal if necessary, for the elucidation of the active site geometry, including the orientation of the molecular oxygen with respect to the two copper centers and protein ligands; and (3) The crystallization of other interesting hemocyanins, such as subunit IIIa, another subunit of Limulus hemocyanin with an active site chemistry much more like a typical molluscan than arthropodan hemocyanin, the whole molecules of Limulus hemocyanin and hybrid and cooperative Limulus hexamers in forms suitable for x-ray diffraction analysis. |
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| NSF Org: |
MCB - Division of Molecular and Cellular Biosciences |
| Award Number: |
9004561 |
| Award Instrument: |
Continuing grant |
| Program Manager: |
Arthur Kowalsky
MCB Division of Molecular and Cellular Biosciences
BIO Directorate for Biological Sciences
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| NSF Program(s): |
CROSS-DIRECTORATE PROGRAMS, MOLECULAR BIOPHYSICS, SPECIAL PROGRAMS-RESERVE, MOLECULAR BIOPHYSICS
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| Field Application(s): |
Health, Life Science Biological |
| Program Element Code(s): |
9120
MOLECULAR BIOPHYSICS, 1164
SPECIAL PROGRAMS-RESERVE, 9145
, 1164
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